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S3 C and S5 Calthough because orientation was not referenced to the polarization excitation, θ d was meaningless. Actin retrograde flow actively aligns and orients ligand-engaged integrins in focal adhesions. Dissection of molecular assembly dynamics by tracking orientation and position of single molecules in live cells. Livsmedel   Recept   Träning   Medlemmar   Dagböcker. Vi som tränar gillar mat med keso protein. For the oil-immersion objective of 1.

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Shi X, et al. Because the measured dipole orientation is a 2D projection on the image plane of a dipole oriented in 3D, multiple orientations of αV-GFP—constrained can lead to a projection compatible with the measured orientation. Direction of actin flow dictates integrin LFA-1 orientation during leukocyte migration. When fluorophores are not aligned, C is a robust measure of angular rotation of fluorophores. This showed that anisotropy was low throughout the basal cell surface Fig. Integrins are transmembrane receptors that, upon activation, bind extracellular ligands and link them to the actin filament F-actin cytoskeleton to mediate cell adhesion and migration. The anisotropic intracellular mechanical environment and shape of FAs may underlie FA sensitivity to directional mechanical cues.

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